홈 > 신규주정보
신규주정보
| 미생물명 | Salicibibacter kimchii NKC1-1 | KCCM 번호 | 43276 |
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| 작성일 | 2018-10-31 | 조회수 | 8274 |
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▷ History
:< ▷ 특성 Kimchi, Gwangju(2014) ▷ 배양정보 - NATRONOBACTERIA MEDIUM KH2PO4, 1.0 g KCl, 1.0 g NH4Cl, 1.0 g MgSO4.7H2O, 0.24 g CaSO4.2H2O, 0.17 g Trace element solution SL-10 (see below)*, 1.0 ml Agar(if necessary), 20.00 g NaCl, 200.0 g Na2-glutamate, 1.0 g Yeast extract, 5.0 g Casamino acids, 5.0 g Na2CO3, 5.0 g Add distilled water to give a final volume of 1000 ml. Adjust pH to 6.5 before autoclaving! Sterilize Na2CO3 separate from medium, add after cooling. Check final pH to be 9.0 - 9.5. If agar medium is prepared, heat and dissolve agar before adding sodium chloride. *Trace element solution SL-10: HCl (25%; 7.7 M), 10.0 ml FeCl2.4H2O, 1.5 g ZnCl2, 70.0 mg MnCl2.4H2O, 100.0 mg H3BO3, 6.0 mg CoCl2.6H2O, 190.0 mg CuCl2.2H2O, 2.0 mg NiCl2.6H2O, 24.0 mg Na2MoO4.2H2O, 36.0 mg Distilled water, 990.0 ml First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml. ▷ 배양온도 : 37℃ |
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